Release of major peanut allergens from their matrix under various pH and simulated saliva conditions—Ara h2 and ara h6 are readily bio-accessible

Auteurs Stef J. Koppelman , Mieke Smits , Monic Tomassen , Govardus A.H. de de Jong , Joe Baumert , Steve L. Taylor , Renger Witkamp , Robert Jan Veldman , Raymond Pieters , Harry Wichers
Gepubliceerd in Nutrients — Open Access Journal of Human Nutrition
Publicatiedatum 11 september 2018
Lectoraat Innovative Testing in Life Sciences & Chemistry
Soort publicatie Artikel

Samenvatting

from the article: "Abstract: The oral mucosa is the first immune tissue that encounters allergens upon ingestion of food. We hypothesized that the bio-accessibility of allergens at this stage may be a key determinant for sensitization. Light roasted peanut flour was suspended at various pH in buffers mimicking saliva. Protein concentrations and allergens profiles were determined in the supernatants. Peanut protein solubility was poor in the pH range between 3 and 6, while at a low pH (1.5) and at moderately high pHs (>8), it increased. In the pH range of saliva, between 6.5 and 8.5, the allergens Ara h2 and Ara h6 were readily released, whereas Ara h1 and Ara h3 were poorly released. Increasing the pH from 6.5 to 8.5 slightly increased the release of Ara h1 and Ara h3, but the recovery remained low (approximately 20%) compared to that of Ara h2 and Ara h6 (approximately 100% and 65%, respectively). This remarkable difference in the extraction kinetics suggests that Ara h2 and Ara h6 are the first allergens an individual is exposed to upon ingestion of peanut-containing food. We conclude that the peanut allergens Ara h2 and Ara h6 are quickly bio-accessible in the mouth, potentially explaining their extraordinary allergenicity."

Aan deze publicatie werkten mee

  • Mieke Smits | onderzoeker | lectoraat Innovative Testing in Life Sciences and Chemistry
    Mieke Smits
    • Onderzoeker
    • Lectoraten: Innovative Testing in Life Sciences & Chemistry
  • Robert Jan Veldman | onderzoeker | lectoraat Innovative Testing in Life Sciences and Chemistry
    Robert Jan Veldman
    • Onderzoeker
    • Lectoraten: Innovative Testing in Life Sciences & Chemistry
  • Raymond Pieters | lector | lectoraat Innovative Testing in Life Sciences and Chemistry
    Raymond Pieters
    • Lector
    • Lectoraten: Innovative Testing in Life Sciences & Chemistry

Taal Engels
Gepubliceerd in Nutrients — Open Access Journal of Human Nutrition
Jaar en volume 10 1281
Trefwoorden peanut, Arachis hypogaea, allergen, bio-accessibility, saliva

Raymond Pieters

Raymond Pieters | lector | lectoraat Innovative Testing in Life Sciences and Chemistry

Raymond Pieters

  • Lector
  • Lectoraat: Innovative Testing in Life Sciences & Chemistry

Innovative Testing in Life Sciences and Chemistry