Comparison of Six Commercial ELISA Kits for Their Specificity and Sensitivity in Detecting Different Major Peanut Allergens

Auteurs Julie Nordlee , Aard de Jongh , Joe Baumert , Raymond Pieters , Steve L. Taylor , Stef J. Koppelman , Shyamali Jayasena , Daniëlle Fiechter , Mieke Smits
Gepubliceerd in Journal of agricultural and food chemistry
Publicatiedatum 17 januari 2015
Lectoraat Innovative Testing in Life Sciences & Chemistry
Soort publicatie Artikel

Samenvatting

Author supplied: "Six commercial peanut enzyme-linked immunosorbent assay kits were assessed for their ability to recover peanut from the standard reference material 2387 peanut butter and also for their specificity in detecting four major peanut allergens, Ara h 1, Ara h 2, Ara h 3, and Ara h 6. The percentage recovery of peanut from peanut butter differed across different kits as well as at different sample concentrations. The highest recovery was observed with the Romer and R-Biopharm kits, while four other kits were found to underestimate the protein content of the reference peanut butter samples. Five of the kits were most sensitive in detecting Ara h 3 followed by Ara h 1, while hardly recognizing Ara h 2 and Ara h 6. The other kit showed the highest sensitivity to Ara h 2 and Ara h 6, while Ara h 1 and Ara h 3 were poorly recognized. Although Ara h 2 and Ara h 6 are known to be heat stable and more potent allergens, antisera specific to any of these four peanut proteins/allergens may serve as good markers for the detection of peanut residues."

Aan deze publicatie werkten mee

  • Raymond Pieters | lector | lectoraat Innovative Testing in Life Sciences and Chemistry
    Raymond Pieters
    • Lector
    • Lectoraten: Innovative Testing in Life Sciences & Chemistry
  • Mieke Smits | onderzoeker | lectoraat Innovative Testing in Life Sciences and Chemistry
    Mieke Smits
    • Onderzoeker
    • Lectoraten: Innovative Testing in Life Sciences & Chemistry

Taal Engels
Gepubliceerd in Journal of agricultural and food chemistry
Jaar en volume 63 6
Trefwoorden Peanuts, Allergies, Testing
Paginabereik 1849-1855

Innovative Testing in Life Sciences and Chemistry